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Lullaby siRNA transfection reagent
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Lullaby siRNA transfection reagent

 

商品号

 产品描述

体积

 Size (number of
transfections1 / μg
of DNA)

 Number of
transfections1 /
96 well plates

 订货电话

LL70500

Lullaby(R) siRNA transfection reagent

500

250

1000

0769-22890558

LL71000

Lullaby(R) siRNA transfection reagent

1000

500

2000

0769-22890558

LL73000

Lullaby(R) siRNA transfection reagent

3 X 1000

1500

6000 

0769-22890558 

1 Number of transfection given for a concentration of 10 nM siRNA.

 

实验步骤 (详细实验步骤请参考网页底部的可下载资料)

  The protocol is very straightforward. Please refer to the tables below for specific amount of the respective
compounds and transfection volume. For instance:
· Use 2 PL of Lullaby(R) reagent for 10 nM siRNA final concentration in a 24-well plate (volume 0.5 mL)
· Use 8 PL of Lullaby(R) reagent for 10 nM siRNA final concentration in a 6-well plate (volume 2 mL)
1) Plate the cells the day before transfection or just before transfection (suspension cells) in your appropriate
tissue culture dish and volume of culture medium as suggested in Table 1(见可下载资料).

2) Dilute the siRNA in 50 or 100 mL of culture medium without serum and supplement (such as DMEM) (see
Table 2 for siRNA dilution procedure). The siRNA optimal concentration required to achieve the best gene
silencing effect depends highly on the cells, target and siRNA sequence; consequently, we suggest to first
test a range of siRNA concentration from 1 to 50nM.  

 3) Dilute Lullaby(R) reagent to 50 or 100 mL with culture medium without serum and supplement (such as
DMEM) (see Table 3 for dilution procedure). The Lullaby(R) reagent (store at +4°C) should have an ambient
temperature and be gently vortexed prior to use. It is important to add first the serum free medium to the
tube and then add carefully the Lullaby(R) reagent directly into the serum free medium without
touching any plastic surface. As a starting point you can use the amount of Lullaby(R) indicated in Table 3,
however we recommend to start your first experiment by testing several doses of Lullaby(R) reagent with
one concentration of siRNA as detailed in Table 4 (见可下载资料)in order to achieve the best effects.    

4) Add the 50 or 100 mL of siRNA-diluted solution into the Lullaby(R) reagent diluted solution tube and mix
immediately 4-5 times by pipetting up and down.
Note: We recommend respecting the order of addition; add the siRNA solution into the Lullaby solution.

5) Incubate the mixture (siRNA / Lullaby(R) reagent) 20 minutes at room temperature 

6) Add the 100 or 200 mL of complexes drop by drop directly onto the cells. The final transfection volumes per
well (culture medium + siRNA / Lullaby(R) reagent mixture) are shown in the Table 3.
Note: For some cells, serum-free condition for the first 3 hours of incubation might lead to better gene
silencing. However, in most assays, siRNA delivery has been realized in culture medium with serum.


7) Cultivate the cells under standard conditions until evaluation of the gene silencing. Depending on the siRNA
amount, the gene targeted and the cell type, assays can be monitored 24 to 96h post-transfection. We
recommend 24h and 72h for RNA and protein knockdown analyses, respectively.
Note: optionally, culture medium can be changed 24 or 48 hours post-transfection.   

            

 

Description:               Lullaby(R) siRNA transfection reagent

RNA interference allows gene silencing in mammalian cells. Specifically designed short RNA duplexes (siRNA)
interact very selectively with their mRNA target triggering its sequence specific degradation and protein
synthesis inhibition. Efficient transfection of these siRNA is a critical step for effective gene silencing. Lullaby(R)
siRNA transfection reagent was specially designed to achieve this goal. The cationic lipids formulation
protects siRNA from extracellular degradation, transport them across cell membranes and efficiently release the
siRNA into cells thanks to a triggered endosomal escape mechanism. In this way, Lullaby(R) formulation gives
reliable higher gene silencing efficiencies in numerous cell types than any other transfection reagent. Moreover,
highly efficient transfection and gene silencing are achievable even with low doses of siRNA.

Principal Lullaby siRNA transfection reagent advantages:

1. Exceptional siRNA delivery efficiency and gene silencing (>90%)
2. Suitable for all siRNA applications (co-transfection, endogenous gene silencing)
3. Effective gene silencing at multiple siRNA concentrations including low doses (<10 nM) – minimize the risk
of off-target effects
4. Rapid and Straightforward procedure (3-step)
5. Powerful across a broad spectrum of cells and confluency (from 20 to 90%)
6. Reliable and reproducible gene knockdown results
7. Serum compatible & Non toxic
8. Highly adapted to high-throughput siRNA screening

 

Technical Documentation资料下载:

点击下载点击下载点击下载

 

 

 

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