In vivo ViroMag
In Vivo Targeted Infection
In vivo ViroMag has been designed to improve and target in vivo viral infection.
This original system combines magnetic nanoparticles and viral vectors that will be confined after injection at the magnetically targeted site. In this way, targeted delivery minimizes systemic distribution, increase efficiency, decreases gene vectors inactivation and reduces toxicity.
This reagent is based on the In vivo MagnetofectionTMtechnology.
High and increased transduction / infection efficiency
Magnetically targeted transduction to specific area
Reduction of virus titer and systemic dissemination
Suitable for all types of virus
Improvement of In vivo transduction
Targeted delivery of viral vector
It has been shown that magnetic nanoparticles enhance adenovirus transduction in vivo after intracerebroventricular injection (i.c.v.). Moreover, infection could be confined and directed towards the magnetic field.
Infection of rat embryo brain with adenovirus
Targeted viral infection
A/ Without in vivo AdenoMag B/ With in vivo AdenoMag
Fig1: High efficiency of In vivo ViroMag for targeted delivery of viral vector after intracerebroventricular injection.
The uterine horns of pregnant rats [embryonic day 15] were exposed, and the third ventricle of each embryo was injected with Fast Green combined with Ad-GFP complexed to AdenoMag. When injected with AdenoMag, a magnet was applied for 30s following injection on one side of the embryo cranium. After injection, the uterine horns were replaced. Pictures show the third ventricle of E17 rat embryo (2 days post surgery). In brains non-exposed to magnet (A), the adenovirus-transduced cells are located on both side of the ventricle; cells are mainly restricted to this region indicating that the injection took place in this part of the brain. In brains exposed to magnetic field (B), the infected cells are located on one side of the ventricle due to the 30 s magnet-application. From Sapet et al. 2011; Pharm Res.
More results for targeted delivery of viral vector
High and increased infection efficiency in hard to transfect conditions
Gene delivery and gene therapy in stomach are hampered by the extreme conditions of pH, the abundance of degradative enzymes, the presence of degraded nutriments and bacteria.
Scherer et al. successfully applied adenoviral vector and ViroMag complexes to the lumen of the stomach of mice for transducing the deep cells of the fundic glands. In this model, a permanent magnet was positioned under the stomach covering the area of the gastric fundus.
Transduction in mouse stomach - Harsh environment
Fig2: High infection efficiency in mouse stomach.
In the absence of a magnetic field yields, gene delivery occurred in only a few transfected cells (A,C), while exposure to a magnet for 20 min produces strong and widespread transgene expression (X-gal staining) in the crypts of the fundic glands 4 days after gene delivery (B,D). The adenoviral Magnetofection applied to the stomach produced strong staining of the crypts of fundic glands.
L, lumen; LP, lamina propria; F, fundic glands; S, submucosa; M, muscularis. 40· magnification (upper panel), 400· magnification (lower panel). From Scherer et al., Gene Therapy., 2002; 9:102-109.
More results for lentiviral transduction in hard-to-transfect conditions
In vivo ViroMag starting kit
Contains 250 uL of In vivo ViroMag + 1 Magnets set
In vivo ViroMag 250 uL
In vivo ViroMag 500 uL
Trial kit In vivo ViroMag
Contains 50 uL of In vivo ViroMag + 1 cylinder magnet (o 10 mm)
Contains 1 extra small cylinder (o 2 mm), 1 small cylinder (o 5 mm), 1 cylinder (o 10 mm) and 1 square (18x18 mm) magnets