Magnetic cell purification and gene delivery in one integrated system
i-MICSTTM Technology (Integrated Magnetic Immuno-Cell Sorting and Transfection / Transduction) is a new platform that allows to genetically modifying cells directly on magnetic cell purification columns.
This technology combines cell isolation and genetic modification
in one simple and reliable integrated system and results in efficient and selective gene delivery to target cells.
i-MICSTTM Technology allows you to reduce cell manipulation steps and save time and material.
Isolation and genetic modification in one reliable integrated system
Rapid, simple and ready to use
Suitable for all cells
i-MICSTTM Technology principle
How it works
i-MICSTTM Technology requires:
- Magnetic cell separation systems (not provided by OZ Biosciences)
- Viro-MICSTTM reagent for capturing virus and infecting cells within the magnetic cell
This reagent is a new specific magnetic nanoparticles formulation issued from our MagnetofectionTM technology.
Viro-MICST was developed in association with MACS(R) technology * from Miltenyi Biotec.
Results and demonstration were performed on MS and LS columns with MACS(R) separators
and the cell separation reagents according to MACS(R) cell separation protocol.
Rapid, simple and ready-to-use
Example protocol for transducing 106 cells on a MACS(R)MS column* with a MOI of 1.
I / Cell preparation and Pre-enrichment of the target cells
1. Magnetically label your cells following the manufacturer's instructions
2. If cells to be purified < 50% of total cells, and/or cell purity< 90%, proceed to a
pre-enrichment step on non-modified column(s)